Hepatoprotective properties of flavonoid-enriched lotus seedpod extract
In this study, researchers from Chung Shan Medical University in Taiwan examined the anti-inflammatory effects of lotus seedpod extract (LSE) on lipopolysaccharide (LPS)-induced hepatic inflammation, as well as the underlying mechanisms, in vitro and in vivo. Their results were published in The American Journal of Chinese Medicine.
- The endotoxin LPS plays an important role in accelerating hepatic inflammation.
- On the other hand, medicinal plants that can prevent inflammation by targeting LPS have potential therapeutic clinical application.
- LSE is a traditional Chinese herbal medicine used to induce hemostasis and eliminate bruises.
- Using an LPS-stimulated human hepatoma cell line (HepG2), LSE and its purified compound (-)-epigallocatechin (EGC) dose-dependently inhibited the expression of pro-inflammatory cytokines and mediators without affecting cell viability. These included tumor necrosis factor (TNF)-a, interleukin (IL)-6, cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (iNOS).
- Molecular studies also revealed that the anti-LPS effect of EGC can be mediated via down-regulation of Toll-like receptor 4 (TLR4). TLR4 mediates both NF-kB and p38 signaling, as proven by treatment with pyrrolidine dithiocarbamate (PDTC), a specific NF-kB inhibitor.
- In vivo, the researchers found that LSE significantly ameliorated LPS-induced hepatic inflammation in treated mice.
- LSE dose-dependently reduced serum levels of biochemical markers of liver damage and inhibited changes in hepatic lobular architecture, the secretion of pro-inflammatory mediators and the induction of antioxidant enzymes.
Based on these results, the researchers concluded that EGC-enriched LSE is an anti-inflammatory agent that can be used for the treatment of hepatic inflammation.
Tseng HC, Tsai PM, Chou YH, Lee YC, Lin HH, Chen JH. IN VITRO AND IN VIVO PROTECTIVE EFFECTS OF FLAVONOID-ENRICHED LOTUS SEEDPOD EXTRACT ON LIPOPOLYSACCHARIDE-INDUCED HEPATIC INFLAMMATION. The American Journal of Chinese Medicine. 2019;47(01):153–176. DOI: 10.1142/s0192415x19500083